ANSWER: Yes. You can see the result of two-step lithography using acetate mask in the photo above. The acetate mask is an inexpensive solution and can provide great results for many patterns over 100 microns.
However, acetate mask has a few weaknesses:
1. The patterns you have designed cannot be printed very smoothly as in the chrome mask (For example, circles can come out like a daisy ☺).
2. Acetate has micron-sized air bubbles in its structure. They can also allow some UV light.
3. In two-step lithography, when you switch to the vacuum stage after the alignment process, your alignment can be slightly distorted because acetate is a flexible material.
But, as seen in the photo, you can get very good results even at 70 microns when you try. Of course, the capabilities of our UV-EX Pioneer mask aligner system help a lot.